Troubleshooting Immunofluorescence Assays
Problem: Antigen or substrate cells smeared or absent from wells before test. Possible Causes:
1. Substrate slides must be allowed to equilibrate with ambient temperature prior to being opened. Opening cold slide packets will quickly lead to moisture condensation and produce an antigen suspension in the condensate liquid. Such slides are not useable. Allow at least 15-20 minutes for temperature equilibration, with the slides individually laying on the bench surface (not stacked).
2. Substrate slides are routinely checked for vacuum leakage prior to shipping. If you have received slide packets that are not flat beyond the edges of the substrate slide, please notify your supplier.