|Troubleshooting Immunofluorescence Assays
|Question: What reaction density (bacteria) constitutes a positive reaction? Answer:
|Antigen density in each antigen spot must be compared with the Positive Control reaction. Fluorescent particles may be seen within antigen spots and elsewhere in the open space, yet the true positive reaction much appear in density like the Positive Control, which is the criterion for judging a particular well as positive.
IFA Frequently Asked Questions