Ehrlichia are zoonotic obligate intracellular alpha-proteobacteria of the family Anaplasmataceae. Ticks serve as as the main reservoir and as vectors of ehrlichiosis while feeding on both humans and animals. The antibody response to infections by Ehrlichia chaffeensisEhrlichia ewingii, Ehrlichia muris and Ehrlichia canis is primarily directed against outer-membrane protein antigens and is most often detected using standard IFA and MIF methods. Custom formats containing combinations of these Ehrlichia or Anaplasma antigens can be made available. Call or email your needs.

Our IFA slides contain infected cells in a traditional format, while our 2-antigen MIF (depicted below) pairs purified Anaplasma phagocytophilum with purified Ehrlichia chaffeensis (or Ehrlichia canis) in each slide well.

The MIF format removes the vast majority of host cell cytoplasmic, nuclear and membrane constituents that are common sources of “non-specific” fluorescence. To produce a distict marix for these antigens, we use a sonicated chicken cell suspension that absorbs the Evans’ blue counterstain. The goal of this approach is to produce a reaction that can be immediately classified as either positive or negative, saving valuable technician time and avoiding erroneous results cause by irrelevant fluorescence.

Anaplasma Slide

 EC-12  Ehrlichia canis 12-well IFA Substrate Slides  ASR
 ECH-12  Ehrlichia chaffeensis 12-well IFA Substrate Slides  ASR
 EM-12  Ehrlichia muris 12-well IFA Substrate Slides  ASR
 EC-GC  Ehrlichia canis IFA Canine Control Set
 E20-12  Ehrlichia canis / Anaplasma 2-antigen MIF Slides
 CG-25  Working Canine IgG Conjugate, 2.5 mL  ASR
 MSD-10  MIF IgG Serum Diluent, 10 mL  ASR